The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.
Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists. These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.
We encourage you to explore our facility as well as other Core Facilities on our campus.
Leica TCS SP5 Spectral Confocal Microscope
Nikon A1R Confocal & N-SIM Super Resolution System
Nikon Eclipse TE300 Widefield Microscope
Zeiss AxioObserver Z1 Widefield/Apotome Microscope
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope
BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter
BD Biosciences FACSCalibur Flow Cytometer
BD Biosciences LSRII Flow Cytometer
BD Biosciences LSRII-SORP Flow Cytometer
Affymetrix GeneChip System
Laser Capture Microdissection
Arcturus XT LCM System
Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer
Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument
Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer
Waters SYNAPT High-Resolution Mass Spectrometer
Waters Q-TOF Micro Mass Spectrometer
Equipment Description & Usage
REAL-TIME QUANTITATIVE PCR
Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)
The RCF currently houses three Bio-Rad CFX96 instruments. The CFX96 is a six-channel real-time PCR system that combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection. The system’s solid-state optical technology (six filtered LEDs, each with a corresponding filtered photodiode) maximizes fluorescence detection for specific dyes in specific channels, providing sensitive detection for quantification and target discrimination. Data are collected from all wells during data acquisition. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to sacrifice data collection on one of the channels to normalize to a passive reference. Users can select multiple data acquisition modes, including a one-color fast scan for SYBR green. Thermal gradient features can be used to optimize reactions in a single run. The new CFX Manager software has advanced analysis tools for performing normalized gene expression. In addition, this system does not require fluorescein or ROX for instrument normalization.
The Research Core Facility has one Bio-Rad CFX384 Touch Real-Time PCR System. The system's solid-state optical technology (5 filtered LEDs and 5 filtered photodiodes) provide precise quantification and multiplex target discrimination. All other acquisition and analysis parameters are identical to the CFX96 Fast System.