The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.
Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists. These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.
We encourage you to explore our facility as well as other Core Facilities on our campus.
Leica TCS SP5 Spectral Confocal Microscope
Nikon A1R Confocal & N-SIM Super Resolution System
Nikon Eclipse TE300 Widefield Microscope
Zeiss AxioObserver Z1 Widefield/Apotome Microscope
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope
BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter
BD Biosciences FACSCalibur Flow Cytometer
BD Biosciences LSRII Flow Cytometer
BD Biosciences LSRII-SORP Flow Cytometer
Affymetrix GeneChip System
Laser Capture Microdissection
Arcturus XT LCM System
Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer
Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument
Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer
Waters SYNAPT High-Resolution Mass Spectrometer
Waters Q-TOF Micro Mass Spectrometer
Equipment Description & Usage
Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)
The Illumina MiSeq is a next-generation DNA sequencer with a single-lane flow cell. It is capable of generating up to 25 million reads with up to 15GB of output in a single run. It can produce 2 x 300 paired end reads. It has the output to accommodate targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression and amplicon sequencing. Sample libraries are loaded directly onto the MiSeq where amplified clusters are generated on the flow cell, followed by sequencing by synthesis. Samples can be barcoded and multiplexed or pooled together in the same lane.
Illumina NextSeq 550
The Illumina NextSeq 550 is a high-throughput sequencer that uses sequencing by synthesis technology, a highly accurate reversible-terminator technology. This technology uses fluorescently labeled nucleotides to sequence hundreds of millions of clusters on a flow cell surface in parallel. This method greatly reduces the number of errors and missed calls associated with homopolymers. The NextSeq 550 integrates cluster generation and sequencing into a single instrument, generating base calls and quality metrics in real time. Data are automatically transferred to the BaseSpace environment for simplified analysis, storage, and sharing. The NextSeq 550 is flexible, offering tunable read lengths and output options to support individual research needs. This system has a maximum output of up 120GB with up to 400 million sequencing reads. Applications include genomic DNA sequencing, exome sequencing, mRNA sequencing, small RNA/microRNA sequencing, epigenetics, small genome sequencing and targeted resequencing.