The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.
Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists. These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.
We encourage you to explore our facility as well as other Core Facilities on our campus.
Leica TCS SP5 Spectral Confocal Microscope
Nikon A1R Confocal & N-SIM Super Resolution System
Nikon Eclipse TE300 Widefield Microscope
Zeiss AxioObserver Z1 Widefield/Apotome Microscope
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope
BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter
BD Biosciences FACSCalibur Flow Cytometer
BD Biosciences LSRII Flow Cytometer
BD Biosciences LSRII-SORP Flow Cytometer
Affymetrix GeneChip System
Laser Capture Microdissection
Arcturus XT LCM System
Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer
Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument
Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer
Waters SYNAPT High-Resolution Mass Spectrometer
Waters Q-TOF Micro Mass Spectrometer
Equipment Description & Usage
Scientific Advisor: Dr. Matthew Woolard
Flow Cytometry Research Specialist: David Custis, MS
BD Biosciences FACSAriaIII
The FACSAriaIII cell sorter is capable of 17-parameter (Forward Scatter, Side Scatter, and 15 fluorescence detectors) analysis and cell sorting. It has four solid-state lasers for excitation at wavelengths of 405 nm, 488 nm, 561 nm and 633 nm. This is a digital high-speed sorter, capable of sorting up to 70,000 events per second. It is capable of standard “bulk” sorting of up to four user-defined cell populations simultaneously, or can be used for direct deposition of a counted number of cells directly into tissue culture plates for cloning, frequency response assays, or other single cell analyses. This instrument uses BD Biosciences FacsDiva software for acquisition and analysis.
BD Biosciences FACSCalibur
The RCF maintains a FACSCalibur flow cytometer available for investigator use. Documented training by staff is required for its use. The FACSCalibur is an ultra-sensitive flow cytometer, capable of six parameter (two laser light scatter and up to four fluorescent colors) analysis. It uses two lasers for fluorochrome excitation: an Argon ion laser for 488 nm excitation and a Red Diode laser for 635 nm excitation. This instrument uses CellQuest Pro software for acquisition and analysis.
BD Biosciences LSRII-UV
This LSRII is capable detecting up to 14 parameters (Forward Scatter, Side Scatter, and twelve fluorescence detectors). It has four solid-state lasers for excitation at wavelengths of 355 nm, 405 nm, 488 nm, and 633 nm. This instrument uses BD Biosciences FacsDiva software for acquisition and analysis.
BD Biosciences LSRII-SORP
This LSRII is capable of detecting up to 17 parameters (Forward Scatter, Side Scatter, and fifteen fluorescence detectors). It has four solid-state lasers for excitation at wavelengths of 405 nm, 488 nm, 561 nm and 633 nm. This instrument uses BD Biosciences FacsDiva software for acquisition and analysis.
Flow Cytometry Workstations and Software
There is a Macintosh MacPro and two Dell PCs for off-line analysis of data. The off-line Macintosh computer is loaded with Cellquest Pro, while the off-line PCs are loaded with the latest version of FacsDiva software and FCS Express for data analysis. In addition, FlowJo is available on the Macintosh and Dell workstations for specific data analysis needs. For the analysis of cell cycle data, ModFit LT is available on both the Macintosh and PC workstations.