Zeiss LSM Confocal & Two-Photon System
The Zeiss LSM 510 NLO system is configured to enhance living tissue and animal research. The scanning system is connected to an upright Axioskop 2 FS MOT microscope with a set of objectives selected for physiological measurements and live animal studies. The stage remains in a fixed position, and the objectives have motorized focus control. The microscope is equipped with 6 laser lines for confocal imaging and a Coherent IR laser for 2-photon imaging.
Capability
Bright field and fluorescence imaging
Two-photon fluorescence imaging
2D, 3D, and 4D image collection and processing, 3D/4D animation, calibration and measurement of ion concentration, time series analysis, and graphical mean ROI analysis.
Lasers
Argon (458, 477, 488, 514 nm),
HeNe (543nm),
HeNe (633nm),
Coherent Chameleon-XR Ti:Sapphire laser (tunable from 705 through 980nm).
Samples
The ultrafast pulsed Chameleon laser emitting NIR radiation allows imaging up to 500 μm deep within tissue.
Tissue slides may reach 1 mm thick.
Objectives
|
Obj |
Objective |
Medium |
|---|---|---|
|
10x |
EC Plan-Neofluar 10x/0.3 |
Dry |
|
20x |
Achroplan 20x/0.5 W |
Water |
|
20x |
LD Plan-Neofluar 20x/0.4 Ph2 Korr |
Dry |
|
25x |
LCI Plan-Neofluar 25x/0.8 oil DIC ∞0-0.17 Glyc-w Imm Korr |
Glycerin and Water |
|
40x |
W Plan-Apochromat 40x/1.0 DIC VIS-IR |
Water |
|
40x |
C-Apochromat 40x/1.2 W Korr UV-VIS-IR ∞0.14-0.19 |
Water |
|
40x |
IR-Achroplan 40x/0.80 W |
Water |
|
63x |
IR-Achroplan 63x/0.90 W |
Water |
|
63x |
W Plan-Apochromat 63x/1.0 VIS-IR |
Water |
|
63x |
Plan-Apochromat 63x/1.4 oil DIC ∞/0.17 |
Oil |
Detectors
Three PMTs for visible wavelength detection
A transmitted light detector
Two non-descanned (ND) detectors for multiphoton imaging.
Software
The operating software is ZEN 2009 that permits 2D, 3D, and 4D image collection and processing, 3D/4D animation, calibration and measurement of ion concentration, time series analysis, and graphical mean ROI analysis.
CONTACT US
Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Email: Malgorzata.Bienkowskahaba@lsuhs.edu
Phone: (318) 675-8537
Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Associate Professor, Neurology
hungwen.lin@lsuhs.edu
(318) 675-8810