Zeiss AxioObserver & Apotome
The system is built with a Zeiss Axio Observer Z1 inverted fluorescent microscope, fully automated with component recognition to minimize errors. System components include mercury arc lamp excitation, a Zeiss AxioCamMRm CCD camera with 12-bit dynamic range and extended sensitivity in the near infrared, fully automated xyz stage, a complement of objectives from 10x through 100x, 5 installed filter sets for DAPI, FITC, narrow band GFP, Rhodamine, and far red. The Apotome attachment is designed for precise optical sectioning. The Apotome projects a grid onto the image plane, which allows 3 raw images taken. A software algorithm then removes out-of-focus signals based on the 3 raw images.
CapabilityBright field (Phase contrast, DIC) |
Sample Size
Apotome optical sectioning: Best for samples no more than 20 microns thick.
Objectives
| Obj | Objective | Medium | Grid |
|---|---|---|---|
| 10x | EC Plan-Neofluar 10x/0.3 DIC I | Air | L |
| 20x | Plan-Apochromat 20x/0.8 DIC II | Air | L |
| 40x | LD Plan-Neofluar 40x/0.6 Ph2 DIC II | Air | NA* |
| 40x | Plan-Apochromat 40x/1.3 DIC (UV) VIR-IR | Oil | L |
| 63x | Plan-Apochromat 63x/1.4 DIC III | Oil | H |
| 100x | Plan-Apochromat 100x/1.4 | Oil | D |
Filters
| Cube | Turret Position | Ex Filter | Dichroic (FT) | Em Filter | Quote Name |
|---|---|---|---|---|---|
| Brightfield | 1 | ||||
| 34 | 2 | 390/22 | 420 | 460/50 | BLUE GFP |
| 38 HE | 3 | 470/40 | 495 | 525/50 | EGFP |
| 31 | 4 | 565/30 | 585 | 620/60 | Cy 3.5 |
| 17 | 5 | 485/20 | 510 | 515/565 | BP |
| 50 | 6 | 640/30 | 660 | 690/50 | Cy 5 |
|
|
CONTACT US
Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537
Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Assistant Professor, Neurology
hlin2@lsuhsc.edu
(318) 675-8810