Olympus CSU W1 Spinning Disk Confocal System

The Olympus SpinSR is a high-resolution and fast spinning disk confocal system built on an inverted IX83 microscope base, with fully automated objectives, stage, and filter turrets. The microscope is equipped with two cutting-edge sCMOS cameras (Hamamatsu Fusion) for simultaneous acquisition on two channels. The Yokogawa CSU W1 spinning disk unit houses two disks (25  and 50 µm ) to best fit different objectives. The system is connect to a feedback stage-top incubation chamber for CO2, and humidity control.


Reservation Calendar

Capabilities

Fixed and live sample imaging
Bright-field, DIC, and fluorescence imaging
Multipoint acquisition, Multichannel acquisition, Large image scan (image stitching), Z-stack, and 2D/3D deconvolution.

Strength

The Hamamatsu Fusion cameras achieve very low and homogeneous background when images are acquired. This system is specifically designed for fast imaging to capture dynamic biological events in real-time. It is also exceptional for large image scan due to its fast speed. Mean while, the system is loaded with high NA objectives to provide excellent resolution while imaging at fast speed.

ZDC: The Z drift correction function combats focal drift and maintain long term time-lapse focus stability.

Lasers

405nm, 488nm, 561nm, 640nm

Samples

Confocal optical sectioning is best for samples less than 200 microns thick

Software

CellSens Dimension software in a Windows 10 operating system.

 

Objectives

Obj

Objective

Medium

Spin Disk Use

 4x UPlan FL N 4x/0.13NA Dry 25, 50 μm

10x

UPlan FL N 10x/0.30NA

Dry

25, 50 μm 

20x

UPlan Apo
20x/0.80NA 

Dry

25, 50 μm

40x

UPlan Apo 40x/1.40NA

Oil

50 μm

60x

UPlan Apo 60x/1.50NA

Oil

50 μm

100x

UPlan Apo
100x/1.50NA 

Oil

50 μm

 

CONTACT US

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Assistant Professor, Neurology
hlin2@lsuhsc.edu
(318) 675-8810