Instrumentation

The Thermo Scientific™ Orbitrap Exploris™ 480 Mass Spectrometer is a quadrupole-Orbitrap™ mass spectrometer which provides high-resolution, accurate-mass (HRAM) data, generating incredibly high resolution measurements of up to 480,000 FWHM at m/z 200.  Due to its HRAM capabilities, it can be used for low-abundance, high complexity samples at sub-ppb concentrations and /or sub-ppm mass differences. With the option to extend the mass range up to m/z 8000, it facilitates our in-depth characterization needs from intact, top and middle-down through to peptide mapping.

In addition, it is combined with Thermo Scientific™ UltiMate™ 3000 RSLCnano System and HPG-3200RS PUMP, which has a wide flow-pressure footprint with nano-, capillary-, micro-, and regular-flow options.

The LCQ-DECA XP is an ion-trap mass spectrometer with a dedicated Thermo HPLC system for highly selective and sensitive detection of metabolites and peptides. The instrument’s ion trap mass analyzer is particularly useful in cases where the molecular mass of the analyte is completely unknown. While enabling MSn capabilities, the ion trap provides structural information, allowing the analyst to “Top-down” the structure of the molecule. It is coupled to a Michrom Paradigm MG4 multidimensional LC system (Thermo) with integrated auto sampler. Finally, data dependent scanning allows the user to identify protein/peptide modifications.

The Q-TOF MICRO is a quadrupole/time-of-flight mass spectrometer for MS/MS-based sequencing of peptides with a mass resolving power of ~8,000FWHM. The Q-TOF is interfaced to a Waters CapLC HPLC system with integrated auto sampler for automated unattended nLC-MS/MS. The Q-TOF mass analyzer is particularly useful for the identification of post-translational modification.

The SYNAPT HDMS is capable of operating in both quadrupole time-of-flight (Q-TOF) and ion mobility mode and is primarily used for unbiased detection of peptides and metabolites for proteomics and metabolomics applications, respectively. A nanoAcquity UPLC system with 2D technology is directly interfaced to nanospray ionization source of SYNAPT HDMS for proteomics applications. An Acquity UPLC system is interfaced to ESI/atmospheric pressure chemical ionization (APCI) source for metabolomics applications.  An atmospheric pressure photoionization (APPI) source is also available for the Synapt HDMS. 

The XEVO TQ is a tandem quadrupole mass spectrometer with a dedicated acquity UPLC system for highly selective and sensitive detection of metabolites and peptides. Furthermore, ScanWave technology of Xevo TQ provides enhanced spectral LC/MS/MS data acquisition capabilities.