Real-Time PCR

This is an image of fragmented DNA on an agarose gel.
Fluorescence-based real-time PCR is an established method for precise, efficient and rapid nucleic acid detection. It combines amplification and detection in a single step. The sensitivity of this method permits reliable detection of small amounts of starting template. Because the PCR product is detected using fluorescent dyes in real time, it is quantitative without  requiring the use of gel electrophoresis.   
 
Real-Time PCR Key Applications:
  • Gene expression analysis
  • microRNA and non-coding RNA analysis
  • SNP analysis
  • Genotyping/allelic discrimination

Instrumentation

CONTACT US

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174
 

Rona Scott, PhD
Director, CMTV Genomics Core
Associate Professor, Department of Microbiology and Immunology
rscott1@lsuhsc.edu
(318) 675-6263