Next Generation Sequencing

Next generation sequencing services are available to the research community through the Genomics Core. The concept behind NGS technology is similar to sequencing by capillary electrophoresis – bases of a small DNA fragment are sequentially identified from signals emitted as each fragment is re-synthesized from the template strand. NGS extends this process across millions of reactions in a massively parallel fashion, rather than being limited to a single or a few DNA fragments.
 
This advance enables rapid sequencing of large stretches of DNA base pairs spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run (“An Introduction to NGS,” 2013 - retrieved from Illumina.com). Successful Illumina sequencing is not only dependent on the quality of the DNA or RNA submitted, but also on the quality of the library created and in the selection of the appropriate protocol. Our highly experienced specialists consistently produce uniform libraries for optimal cluster generation and maximal data output.  

CONTACT US

Because of the cost, complexity of the process, and pros and cons of each option, careful thought should be given to the experimental design for your specific research goal. Please contact us for a consultation to ensure optimal experimental design and setup.

 

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174
 

Rona Scott, PhD
Director, CMTV Genomics Core
Associate Professor, Department of Microbiology and Immunology
rscott1@lsuhsc.edu
(318) 675-6263