Laser Capture Microdissection

This is an image of the Arcturus Laser Capture Microdissection system in the Research Core Facility at LSU Health in Shreveport.

The Arcturus XT Laser Capture Microdissection (LCM) Instrument provides an automated approach to laser microdissection of individual cells or multi-cellular structures from slides containing tissue sections. It consists of a Nikon Eclipse Ti-E inverted microscope with a solid-state near IR laser. 2x, 10x, 20x, and 40x objectives are available for use. The stage is formatted to accept up to three 1"x 3" slides.

Principles of LCM:  Laser capture microdissection (LCM) is a method used to separate individual cell types from within complex tissues.  Specifically developed caps coated with a thermoplastic film are placed on the region of interest.  The Arcturus XT instrument is then used to direct a low-powered infrared laser through the cap to melt the film onto the cells of interest.  These cells adhere to the cap when it is lifted from the tissue section – thus, capturing them for further analysis.  The laser does not affect the tissue sample; therefore, the quality of the nucleic acids and proteins within the sample are not compromised.

CONTACT US

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174
 

Rona Scott, PhD
Director, CMTV Genomics Core
Associate Professor, Department of Microbiology and Immunology
rscott1@lsuhsc.edu
(318) 675-6263