Olympus FVMPE-RS Multiphoton Microscope

The Olympus FVMPE-RS multiphoton imaging system is purpose-built for deep imaging in biological tissue, aimed at revealing both detail and dynamics. It is an upright microscope with gantry frame, optimized for intravital imaging with a large space for live animals and experimental equipment. Innovative features for efficient delivery and detection of photons in scattering media enable high signal-to-noise ratio acquisition. The system is equipped with a dual wavelength laser with a tunable range from 680 nm to 1300 nm and a fixed wavelength line at 1045 nm (InSight X3 Dual-OL by Spectra-Physics). Hybrid scan unit consists of a high-speed acquisition resonant scanner and a high-definition galvanometer scanner. High-sensitivity GaAsP detectors and Olympus dedicated multiphoton objectives allow for deep tissue imaging under in vivo and cleared tissue conditions. 

 

Reservation Calendar

   

Capability

Fixed samples and live animals
Two-photon fluorescence imaging
2D, 3D, and 4D image collection and processing, 3D/4D animation

Lasers

Tunable 680-1300nm
Fixed 1045nm

Samples 

Fixed tissue slices and cleared organ samples (up to 4mm), Organoid / spheroid cultures, embryos, small animals (up to 1.5mm)

Filter Cubes for Sample Check with Wide-field Fluorescence

DAPI (350/50X, BS400, 460/50M)
EGFP/FITC/CY2 (470/40X, BS495, 525/50M)
CY3/TRITC (545/25X, BS565, 605/70M)
CY5 (620/60X, BS660, 700/75M)

Available Fluorescence NDD Filter Cubes for Detectors

FV30-FVG - VIOLET (BA410-460) /485/ GREEN (BA495-540)
FV30-FCY – CYAN (BA460-500) /505/ YELLOW (BA520-560)
FV30-FGR – GREEN (BA495-540) /570/ RED (BA575-645)
FV30-FRCY5 – RED (BA575-645) /650/ FAR RED (BA660-750)

Non-Descanned Detectors (NDD)

2 multi-alkali PMT
2 Gallium-Arsenide-Phosphide (GaAsP) PMT 

   

 Olympus FVMPE-RS Manual 
 Coming Soon! 

Objectives

  Objective MAG NA WD (mm) IR Medium
UPLFLN4X  U Plan Fluorite 4X Objective 4x 0.13 17 N/A dry
UMPLFLN10XW LWD PLAN FLUOR 10X WTR DIPPING OBJ 10x 0.3 3.5 yes water

UMPLFLN20XW

LWD PLAN FLUOR 20X WTR DIPPING OBJ 20x 0.5 3.5 yes water
LCPLN20XIR LWD/CC PLAN 20X OBJ 20x 0.45 8.3 near IR dry

LUMPLFLN40XW

LWD PLAN FLUOR 40X WTR DIPPING OBJ 40x 0.8 3.3 yes water

LUMPLFLN60XW

LWD PLAN FLUOR 60X WTR DIPPING OBJ 60x 1.0 2.0 yes water
FV30-AC25W truResolution 25x water immersion objective with a motorized correction collar 25x 1.05 2.0 yes water

   

Available Primary Emission Beam Splitters for Detectors

DICHROIC CUBE DETECTORS CONFIGURATION
FV30-SDM-M - for 2 detectors all emission to RNDD 3/4 (GaAsP)
FV30-SDM570 - for 4 detectors

>570nm RNDD 1/2 (alkali)
<570nm RNDD 3/4 (GaAsP)

Empty/out of light path - for 2 detectors all emission to RNDD 1/2 (alkali)

Contact Us

Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537

Kelly Tatchell, PhD
Scientific Adviser, Microscopy
Professor and Chairman of Biochemistry & Molecular Biology 

Kelly.Tatchell@lsuhs.edu
(318) 675-5163