Monica Cartelle Gestal, PhD
Assistant Professor of Microbiology & Immunology
Bachelor of Science, Biology (1999) - University of A Coruna, Spain
Master of Science, Microbiology and Research (2002) - University of A Coruna, Spain
PhD, Microbiology (2006) - University of A Coruna, Spain
Post-Doctoral Fellow, Marie Curie Intra-European Fellowship (2013), Nottingham University, UK
Post-Doctoral Associate (2019) - University of Georgia
Latest Lab News
Dr. Gestal is editor of two upcoming Special Issues in the journals Microorganisms and Diseases.
Special issue 1
Microorganisms (MDPI publishing group): "Infectious Diseases, New Approaches to Old Problems"
Infectious diseases are one of the leading causes of death worldwide. Past and current efforts focus on its prevention and treatment, aiming to reduce the morbidity, mortality, and the associated economic burden. With the appearance of antibiotics, life expectancy has significantly increased. However, due to the increase in antibiotic resistance, the number of deaths caused by infectious diseases is on the rise, creating the need for alternative therapies. One common trait to most pathogens is their ability to modulate, manipulate, and escape host immune responses. Therefore, immunomodulation approaches, such as adjuvants, therapies, and vaccines, appear to be the most compelling methods to enhance an immune response, and appear to be highly promising.
This aim of this Special Issue is to unite several fields of research to approach the problem from the One Health perspective. This Special Issue emerges as a result of the lack of strategies and solutions to overcome infectious diseases, and as a platform to provide novel strategies for vaccine and therapeutic development, focusing on immunomodulation. https://www.mdpi.com/journal/microorganisms/special_issues/idna
Special issue 2
Diseases (MDPI publishing group): "Using State-Of-The-Art Proteomics to Understand Infectious Diseases. Novel Clinical Diagnostics, Biomarkers and Drug Targets"
Infectious diseases continue to be one of the leading causes of death worldwide, and with the exponential increase in antibiotic resistance, we are facing an era in which the WHO expects around 10 million deaths per year by 2050. This situation creates an imperative need for rapid and precise diagnosis, allowing for quick and efficient treatment that can stop the spread of infectious agents. A challenge in medical microbiology is to develop and apply new experimental approaches that allow for the identification of the microbe as well as its susceptibility profile in a time-sensitive manner. A plethora of novel technologies is being adopted in diagnostics laboratories to augment specificity and sensitivity and decrease morbidity and mortality. Genomics, transcriptomics, proteomics, and metabolomics are new tools applied to an old problem but reveal that the opportunities to apply novel technologies in the diagnostic field are limitless. Faster and more accurate clinical diagnosis helps to improve health and provide targeted therapies that can be tailored to an individual patient and a specific pathogen.
This Special Issue will focus on translational mass spectrometry-based approaches applied to infectious diseases. https://www.mdpi.com/journal/diseases/special_issues/infectious_diseases_diagnostics_targets
The Gestal Lab aims to understand how some pathogens manipulate host immune responses in order to increase survival, persistence, and transmission. We focus on respiratory diseases using Bordetella spp. (causative agent of pertussis or whooping cough) as a model. Pertussis disease is increasingly emerging despite a vaccine being available. A whole cell vaccine was introduced on the mid-1940s leading to a drastic reduction in the number of cases. However, due to undesirable secondary effects, this was replaced by an acellular vaccine in the mid-1990s. This vaccine, although safer, was less effective at blocking colonization and transmission creating an imperative need to improve it. In the lab we want to understand the mechanisms that Bordetella spp. utilize to manipulate, escape, and evade host immune response. Using the powerful in vivo murine model of B. bronchiseptica (which is a natural pathogen of mice), we can study the intricacies of the host-pathogen interactions. Using reverse and forward genetics approaches combined with the mouse immunological tools, our aim is to better understand the signaling mechanisms that orchestrate host immune response.
- Monica C Gestal#, Katie Howard, Kalyan Dewan, Hannah M. Johnson, Mariette Barbier, Illiasou Hamidou, Israel Rivera, Bodo Linz, Clare Bryant, and Eric Harvill. “Transcription Factor-Mediated Immunomodulation Suppresses Innate And Adaptive Immunity To Bordetella Infection”. In revision Scientific communications Sci Rep 9, 20261 (2019) doi:10.1038/s41598-019-56652-z.
- Monica C. Gestal, Hanna M. Johnson, Eric T. Harvill. “Immunomodulation as a Novel Strategy for Prevention and Treatment of Bordetella spp. Infections”. Frontiers in Immunology 10, 2869.
- Keila Belhart1, Maria de la Paz Gutierrez, Federico Zacca, Nicolas Ambrosis, Monica Cartelle Gestal, Dawn Taylor, Kurt Dahlstrom, Eric Harvill, George O'toole, Federico Sisti, and Julieta Fernandez. “Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and is Involved in Transmission Between Hosts.” Journal of Bacteriology JB00011-19
- Kalyan K. Dewan, Dawn L. Taylor-Mulneix, Laura L. Campos , Amanda L. Skarlupka, Shannon M. Wagner, Valerie E. Ryman, Monica C. Gestal, Longhua Ma, Uriel Blas-Machado, Brian T. Faddis, Eric T. Harvill. “A Model for Chronic, Transmissible Otitis Media in Mice”.. PloS Pathogens. 15 (4), e1007696.
- Monica C. Gestal#, Lindsey Whitesides, Eric T. Harvill. ”Integrated Signaling Pathways Mediate Bordetella Immunomodulation, Persistence & Transmission”. Trends Microbiol. 2019 Feb;27(2):118-130. doi: 10.1016/j.tim.2018.09.010. This manuscript also contains a video abstract to be published in Cell Press YouTube Channel.
- Monica C. Gestal, Israel Rivera1, Laura K. Howard2, Kalyan K. Dewan, Illiassou Hamidou Soumana, Margaret Dedloff2, Tracy L. Nicholson, Bodo Linz and Eric T. “Blood or serum exposure induce global transcriptional changes, altered antigenic profile, and increased cytotoxicity by Classical Bordetellae”. Frontiers in Microbiology 07 September 2018, 9:1969. doi: 10.3389/fmicb.2018.01969.
- Michael E. Chirgwin, Margaret R. Dedloff, Alina Maria Holban, and Monica C. Gestal. “Novel Therapeutic Strategies Applied to Pseudomonas aeruginosa Infections in Cystic Fibrosis”. Materials 12 (24), 4093.
- Margaret R. Dedloff, Callie Effler, Alina M. Holban and Monica C. Gestal#. “Use of Biopolymers in Mucosally-Administered Vaccinations for Respiratory Disease”. Materials 12 (15), 2445.
- Monica Cartelle Gestal, Margaret Dedloff and Eva Torres Sangiao. “Computational Health Engineering Applied To Model Infectious Diseases And Antimicrobial Resistance Spread”. Applied Sciences 9 (12), 2486
- Eva Torres-Sangiao, Alina Maria Holban, Monica Cartelle Gestal#. “Applications of Nanodiamonds in the Detection and Therapy of Infectious Diseases”. Materials 2019, 12(10), 1639; https://doi.org/10.3390/ma12101639
- Mara Madalina Mihai, Monica Cartelle Gestal, Alexandru Grumezescu, Alina Maria Holban. “Nanocoatings for wound repair - modulation of microbial colonization and biofilm formation”. International Journal of Molecular Sciences. 2018 Apr 12;19(4). pii: E1179. doi: 10.3390/ijms19041179.
- Torres-Sangiao E3, Holban AM, Gestal MC#. Advanced Nanobiomaterials: Vaccines, Diagnosis and Treatment of Infectious Diseases”. Molecules. 2016 Jul 1;21(7). PMID: 27376260. doi: 10.3390/molecules21070867.
- Grigore ME, Biscu ER, Holban AM, Gestal MC#, Grumezescu AM. “Methods of Synthesis, Properties and Biomedical Applications of CuO Nanoparticles”. Pharmaceuticals. 2016 Nov 30;9(4). pii: E75. PMID: 27916867
- Alina Maria Holban, Monica Cartelle Gestal#, Alexandru Mihai Grumezescu. “Control of biofilm-associated infections by signaling molecules and nanoparticles”. Int J Pharm. 2016 Mar 2. pii: S0378-5173(16)30149-1. PMID: 26945736. doi: 10.1016/j.ijpharm.2016.02.044.
- Andreea Lorena Mateescu, Tatiana Vassu Dimov, Alexandru Mihai Grumezescu, Monica Cartelle Gestal, Mariana Carmen Chifiriuc.“Nanostructured bioactive polymers used in food-packaging”. Current pharmaceutical biotechnology. PMID: 25483716.
- Cartelle Gestal M#, Holban Alina. “Advances in nanotechnology as an alternative against superbugs”. JSM Chem. 2(2): 1011.
- Alexandru Mihai Grumezescu, Monica Cartelle Gestal, Alina María Holban, Valentina Grumezescu, Bogdan Ștefan Vasile, Laurențiu Mogoantă, Coralia Bleotu, George Dan Mogoșanu. “Biocompatible Fe3O4 increases the efficacy of amoxicillin delivery against Gram-positive and Gram-negative bacteria”. Molecules. 2014, 19, 5013-5027 PMID: 24759068. doi: 10.3390/molecules19045013.
- Alina Maria Holban, Monica Cartelle Gestal and Alexandru Mihai Grumezescu. “New Molecular Strategies for Reducing Implantable Medical Devices Associated Infections”. Current Medicinal Chemistry. 2014, Vol 21, 38 PMID: 24606502.
- Alina Maria Holban, Alexandru Mihai Grumezescu, Laurenţiu Mogoantă, Monica Cartelle Gestal, George Dan Mogoșanu. “Novel drug delivery magnetite nano-systems used in antimicrobial therapy”. Current Organic Chemistry. 2014, 18, 185-191 185.
- Dewan, Kalyan; Skarlupka, Amanda; Rivera, Israel; Gestal, Monica; Taylor-Mulneix, Dawn; Wagner, Shannon; Ryman, Valerie; Rodriguez, Coralis; Hamidou Soumana, Illiassou; Levin, Bruce; Harvill, Eric. “Development of macrolide resistance in Bordetella bronchiseptica associated with the loss of virulence”. Journal of Antimicrobial Chemotherapy. 2018 Aug 10. doi: 10.1093/jac/dky264
- Claudia Soria, Glenda Larrea, Monica Cartelle Gestal. “Dissemination of CTX-M-1-Like enzymes in community patients in Ecuador.” Microbial Drug Resistance. Microb Drug Resist. 2018 Jan 3. doi: 10.1089/mdr.2017.0325
- Cartelle Gestal M#, Zurita J, Gualpa G, Gonzalez C, Paz y Mino A. “Early detection and control of an Acinetobacter baumannii multi-resistant outbreak in a hospital in Quito, Ecuador”. J Infect Dev Ctries. 2016 Dec 30;10(12):1294-1298. PMID: 28036308. doi: 10.3855/jidc.7544.
- Monica Cartelle Gestal#; Jeannete Zurita, Ariane Paz y Mino, David Ortega-Paredes, Iliana Alcocer. “Characterization of a small outbreak of Salmonella enterica serovar Infantis that harbour CTX-M-65 in Ecuador”. Braz J Infect Dis. 2016 May 20. pii: S1413-8670(16)30074-5 PMID: 27215783. doi: 10.1016/j.bjid.2016.03.007.
- Claudia Soria, Nelson Nieto, José E. Villacís, Sara Lainez, Monica Cartelle Gestal. “Brote por Serratia marcescens en una Unidad de Cuidados Intensivos Neonatales”. Guayaquil-Ecuador” Rev Chilena Infectol. 2016 Dec;33(6):703-705. PMID: 28146199. doi: 10.4067/S0716-10182016000600016.
- Juan José Romero, Paul Herrera, Mónica Cartelle, Pedro Barba, Jeannete Zurita. “Panniculitis caused by Mycobacterium monacense mimicking erythema induratum: a case in Ecuador”. New Microbes New Infect. 2016 Jan 22;10:112-5. PMID: 26933504. doi: 10.1016/j.nmni.2016.01.006.
- Cartelle Gestal M#, Holban A., Escalante S., Cevallos M. (2015) “Neglected diseases, 20 years of study in Ecuador”. PLoS One. 2015 Sep 22;10(9):e0138311. PMID: 26394405. doi: 10.1371/journal.pone.0138311.
- Jeannete Zurita, Henry Parra, Mónica Cartelle Gestal, Joseph McDermott, Pedro Barba. “First case of NDM-1 producing Providencia retggeri in Ecuador”. Journal of Global Antimicrobial. PMID: 27842879. doi: 10.1016/j.jgar.2015.07.003.
- Cartelle Gestal M# and Escalante S. “New initiative in Ecuador, the creation of a National reference lab for Antibiotic Resistance”. J Develop Drugs. 2014, 3:122
- Ruiz de Alegría C, Rodríguez-Baño J, Cano ME, Hernández-Bello JR, Calvo J, Román E, Díaz MA, Pascual A, Martínez-Martínez L; Spanish Group for Nosocomial Infections (GEIH). “Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases in Spain: microbiological and clinical features”. J Clin Microbiol. 2011 Mar;49(3):1134-6. PMID: 21191059. doi: 10.1128/JCM.02514-10.
- Díaz MA, Hernández-Bello JR, Rodríguez-Baño J, Martínez-Martínez L, Calvo J, Blanco J, Pascual A; Spanish Group for Nosocomial Infections (GEIH). “Diversity of Escherichia coli strains producing extended-spectrum beta-lactamases in Spain: second nationwide study”. J Clin Microbiol. 2010 Aug;48(8):2840-5. PMID: 20519460. doi: 10.1128/JCM.02147-09.
- Ángel Díaz M, Ramón Hernández J, Martínez-Martínez L, Rodríguez-Baño J, Pascual A; Grupo de Estudio de Infección Hospitalaria (GEIH). “Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Spanish hospitals: 2nd multicentre study (GEIH-BLEE project, 2006)”. Enferm Infecc Microbiol Clin. 2009 Nov;27(9):503-10. PMID: 19329230. doi: 10.1016/j.eimc.2008.09.006.
- Fernández JL, Cartelle M, Muriel L, Santiso R, Tamayo M, Goyanes V, Gosálvez J and Bou G. “DNA fragmentation in microorganisms assessed in situ”. Appl Environ Microbiol. 2008. 74:5925-5933. PMID: 18689511. doi: 10.1128/AEM.00318-08.
- Pérez-Llarena FJ*, Cartelle M*, Mallo S, Beceiro A, Pérez A, Villanueva R, Romero A, Bonnet R and Bou G. “Structure-function studies of arginine at position 276 in CTX-M β-lactamases”. J Antimicrob Chemother. 2008. 61:792-797 PMID: 18281307. DOI: 10.1093/jac/dkn031.
- Fernández A, Gil E, Cartelle M, Pérez A, Beceiro A, Mallo S, Tomás M, Pérez-Llarena FJ, Villanueva R and Bou G. “Interspecies spread of CTX-M-32 extended-spectrum ß-lactamase and the role of the insertion sequence IS1 in down-regulating blaCTX-M gene expression”. J Antimicrob Chemother. 2007. 59:841-847 PMID: 17332005. DOI: 10.1093/jac/dkm030.
- Cartelle M, Canle D, Llarena FJ, Molina F, Villanueva R, Bou G. “Characterization of the first CTX-M-10-producing isolate of Salmonella enterica serotype Virchow”. Clin Microbiol Infect. 2006. 12:285-287 PMID: 16451417. DOI: 10.1111/j.1469-0691.2005.01300.x
- Guerrero A, Cañizares A, Tomás S, Velasco D and Cartelle M. “Prevalence of antiretroviral drug resistance among naïve Spanish patients infected with HIV”. Enferm Infecc Microbiol Clin. 2005. 23:605-608 PMID: 16324550.
- Bou G, Figueira M, Canle D, Cartelle M, Eirós JM and Villanueva R. “Evaluation of group B streptococcus differential agar for detection and isolation of Streptococcus agalactiae“. Clin Microbiol Infect. 2005. 11:676-678 PMID: 16008623
- Tomás M, Cartelle M, Pertega S, Beceiro A, Llinares P, Canle D, Molina F, Villanueva R, Cisneros JM and Bou G. “Hospital outbreak caused by a carbapenem-resistant strain of Acinetobacter baumannii: patient prognosis and risk-factors for colonization and infection”. Clin Microbiol Infect. 2005. 11:540-546 PMID: 15966971. DOI: 10.1111/j.1469-0691.2005.01184.x
- Velasco D, del Mar Tomás M, Cartelle M, Beceiro A, Pérez A, Molina F, Moure R, Villanueva R and Bou G. “Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus”. J Antimicrob Chemother. 2005. 55:379-82 PMID: 15722394
- Cartelle M, Tomás M, Pertega S, Beceiro A, Domínguez MA, Velasco D, Molina F, Villanueva R and Bou G. “Risk factors for colonization and infection in a hospital outbreak caused by a strain of Klebsiella pneumoniae with reduced susceptibility to expanded-spectrum cephalosporins”. J Clin Microbiol. 2004. 42:4242-4249 PMID: 15365018. Doi: 10.1128/JCM.42.9.4242-4249.2004
- Cartelle M, Tomás M, Molina F, Moure R, Villanueva R and Bou G. “High level of resistance for ceftazidime conferred by a novel CTX-M enzyme derived from CTX-M-1 through a single Asp-240-Gly substitution”. Antimicrob Agents Chemoter. 2004. 48: 2308-2313 PMID: 15155242. Doi: 10.1128/AAC.48.6.2308-2313.2004
- Velasco D, Pérez S, Peña F, Domínguez MA, Cartelle M, Molina F, Moure R, Villanueva R and Bou G. “Lack of correlation between phenotypic techniques and a PCR-based genotypic method for identification of Enterococcus spp”. Diagn Microbiol Infect Dis. 2004. 49: 151-156 PMID: 15246503
- Gil E, Durán MT, Rodríguez-Tudela JL, Cuenca-Estrella M, Cartelle M, Molina F and Guerrero A. “Pseudozyma sp: colonizer of the respiratory tract in critical patients”. Enferm Infecc Microbiol Clin. 2003. 21:65 PMID: 12550051.
- Bou G, Cartelle M, Tomás M, Canle D, Molina F, Moure R, Eirós JM and Guerrero A. “Identification and broad dissemination of the CTX-M-14 β-lactamase in different Escherichia coli strains in the Northwest area of Spain”. J Clin Microbiol. 2002. 40: 4030-4036 PMID: 12409370. 10.1128/JCM.40.11.4030-4036.2002
Postdocs: We are not actively recruiting postdoctoral fellows, but qualified candidates will be considered. Inquiries should be directed to Dr. Gestal.
Graduate students: Students interested in working in our Department should visit our Graduate Program website: https://www.lsuhscshreveport.edu/departments/school-of-graduate-studies/microbiology-and-immunology/graduate-program.
Undergraduate students: Our Undergraduate Biomedical Research Fellowship program provides bright undergraduates a chance to gain hands-on research experience through a paid internship. More information can be found here: https://www.lsuhscshreveport.edu/departments/school-of-graduate-studies/microbiology-and-immunology/undergraduate-fellowship.