Amoldeep S. Kainth Research

Amoldeep Kainth, PhD

BS, Biotechnology, Panjab University, 2009
MS, Biotechnology, Panjab University, 2011
PHD, Biochemistry & Molecular Biology, LSU Health Shreveport, 2019

Hsf1 is a highly conserved transcription factor that regulates the expression of molecular chaperone (Heat Shock Protein;HSP) genes from yeast to humans. Recent studies from the Gross lab suggest that nuclear organization and chromosome conformation play an important, although as yet mysterious role, in regulating the expression of Hsf1-target genes in model eukaryotic organism, S. cerevisiae (budding yeast). I am investigating molecular mechanisms that underlie the rapid, robust and reversible restructuring and nuclear re-organization of HSP genes that takes place in cells exposed to heat shock.  Specifically, I am addressing two fundamental questions:

1) The role of Hsf1 in regulating HSP gene architecture. Thus far, I have established that Hsf1 is both necessary and sufficient to trigger intergenic (both cis- and trans-) interactions of HSP genes.  

2) Employ a candidate-based approach to identify transcriptional coactivators and chromosomal structural proteins that may act in concert with Hsf1 to govern the 3D architecture of HSP genes. Prime candidates include transcriptional coactivators like Mediator and structural maintenance proteins like cohesin and condensin. Uncovering the roles of these trans-acting factors will afford insights into the mechanistic basis for, and biological relevance of, the dramatic conformational changes in HSP genes that accompany their heat shock-induced transcriptional activation.

Amoldeep S. Kainth Research